Evolutionary Analysis of SARS-CoV-2 Proteome
Select any protein from the SARS-CoV-2 proteome to see evolutionary analysis
Publication: Identification of evolutionarily stable functional and immunogenic sites across the SARS-CoV-2 proteome and the greater coronavirus family
Shows the evolutionary analysis of the selected protein
100-ET Coverage : Importance of each residue in the selected protein. Higher the value (warmer the color) the more important.
EA (Most Frequent Variant) : Evolutionary Action of the most frequent variant at each amino acid position in the current SARS-CoV-2 outbreak.
Higher EA value suggests more functional impact of that substitution
Unique Variants : number of unique amino acid substitution at each position.
All Variants : number of total amino acid substitution at each position.
Amino Acid Position
Selecting the residues for structure viewer
Zoom in to regions of interest by dragging a box over the bar plots or using the residue number input box.
Click “add sequence to structure” to add a selection to the Structure Viewer, which can be highlighted in the protein structure.
Visualization of the evolutionary analysis results on the protein structure. Protein structure is preload with ET color. To focus on a specific region, use the EA/ET Viewer to select regions of interest then drag to the display box. Check Structure Options to map EA, Unique Variants, and All Variants to the structure. Linear and structural epitopes are defined as connected amino acids (linear or spatial) with ET coverage < 30 and not mutated in the current outbreak. They can be selected through the epitope boxes
All selected sequence ranges
Drag and drop the ranges onto structure
The Evolutionary Action (EA) distribution of all variants and unique variants observed in the current outbreak of the selected protein. Higher EA suggest more functional impact for a give substitution. A protein with EA distribution shifting towards higher values suggest it is likely under selection
The EA scores for all possible mutations for the selected protein
*Purple - predicted more neutral mutation
*Red - predicted more deleterious mutation
Selected protein was blasted against Uniref90, Uniref100 and NCBI nr for homology. Sequences that pass our filter were used to run evolutionary analysis.
Files available for download:
Data version: December 2020
|All data files(Includes PyMOL file for selected structure)||All data zip file|
|ET ranks file||protein.ranks|
|EA predicted score||protein.pred|
|nr Alignment file||alignment.nr.fa|
|u90 Alignment file||alignment.u90.fa|
|u100 Alignment file||alignment.u100.fa|
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